Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting-Reference-Cited by-同舟云学术

登录注册会员服务联系我们

Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

Published:2016-08-19 Issue:9 Volume:29 Page:355-366
ISSN:1741-0126
Container-title:Protein Engineering Design and Selection
language:en
Short-container-title:Protein Engineering, Design and Selection

Author:

Obexer Richard,Pott Moritz,Zeymer Cathleen,Griffiths Andrew D.,Hilvert Donald

Funder

Swiss National Science Foundation

Lithuanian-Swiss Research and Development Cooperation Program and ETH Zurich

Marie Skłodowska-Curie Individual Fellowship (TIMEnzyme)

Publisher

Oxford University Press (OUP)

Subject

Molecular Biology,Biochemistry,Bioengineering,Biotechnology

Link

http://academic.oup.com/peds/article-pdf/29/9/355/18547653/gzw032.pdf

Reference43 articles.

1. Iterative Saturation Mutagenesis: A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution

2. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

3. Robust design and optimization of retroaldol enzymes

4. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

5. Exploration of Alternate Catalytic Mechanisms and Optimization Strategies for Retroaldolase Design

Cited by 58 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. An intelligent droplet sorter using optimized liquid-metal electrodes for droplet sorting under a low voltage;Sensors and Actuators B: Chemical;2024-11

2. Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies;Biotechnology Advances;2023-09

3. Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments;Chemical Reviews;2023-05-01

4. Enzyme Cascade with Horseradish Peroxidase Readout for High-Throughput Screening and Engineering of Human Arginase-1;Analytical Chemistry;2023-04-24

5. Rapid quantitative assessment of small molecule leakage from microdroplets by flow cytometry and improvement of fluorophore retention in biochemical assays;2023-04-23

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号京ICP备18003416号-3