Hard Surface Carrier Test for Efficacy Testing of Disinfectants: Collaborative Study

Author:

Rubino Joseph R1,Bauer Janice M1,Clarke Paul H2,Woodward Betsy B3,Porter Fran C3,Hilton H Gill4

Affiliation:

1. L & F Products, One Philips Parkway, Montvale, NJ 07645

2. Stepan Co., 22 W. Frontage Rd, Northfield, IL 60093

3. Florida Department of Agriculture and Consumer Services, 3125 Conner Blvd, Tallahassee, FL 32301

4. Brigham Young University, Center for Statistical Research, Provo, UT 84602

Abstract

Abstract A collaborative study was undertaken to evaluate a new disinfectant efficacy method called the hard surface carrier test. This new method is a qualitative carrier test that uses disposable glass carriers and standardized bacterial cultures. Ten laboratories tested 6 disinfectant-type formulations, which included positive and negative controls, against 3 microorganisms. No significant differences were found among the 10 laboratories for tests with Pseudomonas aeruginosa and Salmonella choleraesuis, but a small but statistically significant difference was present among laboratories for Staphylococcus aureus. The majority of data for this organism showed very good agreement; however, several tests exhibited slightly higher positive responses, which resulted in this overall difference. This difference was not considered significant within the scope and precision of this method or when compared with results for the other 2 organisms. The initial estimates of pure intralaboratory variance, determined from mean squares from analysis of variance results, were 1.009,0.295, and 1.553 for P. aeruginosa, S. aureus, and S. choleraesuis, respectively. The experimental error for S. aureus was 3-5 times smaller than for the other 2 organisms, which helps explain the statistical significance of the results observed with this organism. Final estimates of intralaboratory variance obtained after dropping nonsignificant terms from the models were 0.90,0.30, and 0.58 for P. aeruginosa, S. aureus, and S. choleraesuis, respectively. Small but statistically significant differences were noted in the formulation means for P. aeruginosa and S. aureus but not for S. choleraesuis. The results of this study suggested a performance standard of ≤2 positive carriers out of 60 tested for S. aureus and S. choleraesuis, and ≤3 positive carriers out of 60 tested for P. aeruginosa. This standard was derived from an analysis of the data by calculating an expected count of positive carriers and a 95% upper confidence limit for a set of 60 carriers. The method has been adopted first action by AOAC International.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

Cited by 9 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Hard Surface Carrier Test Methods;Official Methods of Analysis of AOAC INTERNATIONAL;2023-01-04

2. Reproducibility problem with a proposed standard method to measure disinfection efficacy;2022-03-15

3. Reproducibility of antimicrobial test methods;Scientific Reports;2018-08-22

4. Antibacterial Polymers and Coatings;Functional Polymer Coatings;2015-05-22

5. Evaluation of Antimicrobial Efficacy;Russell, Hugo & Ayliffe's;2012-12-17

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