Evaluation of Direct Saponification Method for Determination of Cholesterol in Meats

Abstract

Abstract A gas chromatographic (GC) method has been developed for determination of cholesterol in meats. The method involves (a) ethanolic KOH saponification of the sample material, (b) homogeneous-phase toluene extraction of the unsaponifiables, (c) derivatization of cholesterol to its trimethylsilylether, and (d) quantitation by GC-flame ionization detection using 5-α-cholestane as internal standard. This direct saponification method is compared with the current AOAC official method for determination of cholesterol in 20 different meat products. The direct saponification method eliminates the need for initial lipid extraction, thus offering a 30% savings in labor, and requires fewer solvents than the AOAC method. It produced comparable or slightly higher cholesterol results than the AOAC method in all meat samples examined. Precision, determined by assaying a turkey meat sample 16 times over 4 days, was excellent (CV = 1.74%). Average recovery of cholesterol added to meat samples was 99.8%.