Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid

Author:

Juergens Hannes1,Varela Javier A2,Gorter de Vries Arthur R1ORCID,Perli Thomas1,Gast Veronica J M1,Gyurchev Nikola Y1,Rajkumar Arun S2,Mans Robert1,Pronk Jack T1,Morrissey John P2,Daran Jean-Marc G1ORCID

Affiliation:

1. Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands

2. School of Microbiology/Centre for Synthetic Biology and Biotechnology/Environmental Research Institute/APC Microbiome Institute, University College Cork, Cork T12 YN60, Ireland

Funder

European Union's Seventh Framework Programme

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Microbiology

Reference86 articles.

1. Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus;Abdel-Banat;Yeast,2010

2. Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae;Bao;ACS Synth Biol,2015

3. Timing is everything: cell cycle control of Rad52;Barlow;Cell Div,2010

4. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae;Baudin;Nucleic Acids Res,1993

5. Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance;Boulton;Nucleic Acids Res,1996

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