Evaluation of Nicked Human Chorionic Gonadotropin Content in Clinical Specimens by a Specific Immunometric Assay

Author:

Kovalevskaya Galina1,Birken Steven2,Kakuma Tatsu3,Schlatterer John1,O’Connor John F14

Affiliation:

1. Irving Center for Clinical Research, Columbia University College of Physicians and Surgeons, NewYork, NY 10032.

2. Departments of Medicine, Columbia University College of Physicians and Surgeons, NewYork, NY 10032.

3. Departments of Pathology, Columbia University College of Physicians and Surgeons New York, NY 10032

4. New York Hospital-Cornell Medical Center, White Plains, NY 10605

Abstract

AbstractWe report the development and characterization of an IRMA for the direct measurement of nicked human chorionic gonadotropin (hCGn) in blood and urine. hCGn derived from a reference preparation of hCG used as an immunogen elicits monoclonal antibodies (mAbs) with enhanced recognition of human luteinizing hormone epitopes. The most specific assay for pregnancy hCGn is an IRMA composed of one mAb to choriocarcinoma-derived hCGn (C5) and a second mAb developed from immunization with normal-pregnancy hCGn. This assay was used to evaluate hCGn profiles in normal, in vitro fertilization, Down syndrome, and ectopic pregnancies. In all pregnancies, hCGn was usually present in much lower concentrations than the non-nicked hCG isoform. Our results suggest that some form of physical separation from the overwhelming quantities of non-nicked hCG present in clinical specimens will be required before accurate immunochemical estimations of hCGn can be made.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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