A Method for Measurement of Cholesterol in Blood Serum

Author:

Mann George V1

Affiliation:

1. Departments of Surgery, Biochemistry and Medicine, Vanderbilt University School of Medicine, Nashville, Tenn

Abstract

Abstract A method for measuring cholesterol in 0.1 ml. of blood serum is described. This uses the saponification and extraction procedure of Abell et al. (4) and the color reaction of Zlatkis et al. (7). The procedure is shown to be accurate and reproducible. It is compared with several commonly used procedures and shown to be equal or superior to these. An adaptation of the method to filter-paper stored samples described. The method is recommended for use in clinical laboratories.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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