Highly Sensitive and Specific Fluorescence Reverse Transcription-PCR Assay for the Pseudogene-free Detection of β-Actin Transcripts as Quantitative Reference-Reference-Cited by-同舟云学术

Highly Sensitive and Specific Fluorescence Reverse Transcription-PCR Assay for the Pseudogene-free Detection of β-Actin Transcripts as Quantitative Reference

Published:1999-02-01 Issue:2 Volume:45 Page:297-300
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Kreuzer Karl-Anton¹,Lass Ulrich¹,Landt Olfert²,Nitsche Andreas¹,Laser Jutta¹,Ellerbrok Heinz³,Pauli Georg³,Huhn Dieter¹,Schmidt Christian Andreas¹

Affiliation:

1. Department of Medicine, Division of Hematology/Oncology, Charité-Virchow-Klinikum, Humboldt University Berlin, Augustenburger Platz 1, 13353 Berlin, Germany;

2. TIB Molbiol Inc., 10829 Berlin, Germany;

3. Robert Koch Institute, Department of Virology, 13353 Berlin, Germany

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/45/2/297/32721329/clinchem0297.pdf

Reference7 articles.

1. Differential Expression Patterns of β-Actin mRNA in Cells Undergoing Apoptosis

2. Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes.

3. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

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