Characterisation and validation of housekeeping genes for qRT-PCR expression analysis in Pterophyllum scalare-Reference-Cited by-同舟云学术

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Characterisation and validation of housekeeping genes for qRT-PCR expression analysis in Pterophyllum scalare

Published:2021-07-29 Issue:6 Volume:29 Page:2387-2402
ISSN:0967-6120
Container-title:Aquaculture International
language:en
Short-container-title:Aquacult Int

Author:

Sushila Ngairangbam,Das Basanta Kumar,Prasad K. Pani,Tripathi Gayatri

Publisher

Springer Science and Business Media LLC

Subject

Agronomy and Crop Science,Aquatic Science

Link

https://link.springer.com/content/pdf/10.1007/s10499-021-00754-x.pdf

Reference28 articles.

1. Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245–5250. https://doi.org/10.1158/0008-5472.CAN-04-0496

2. Bao Z, Huang Y, Chen J, Wang Z, Qian J, Xu J, Zhao Y (2019) Validation of reference genes for gene expression normalization in RAW264.7 cells under different conditions. Biomed Res Int 2019:9. https://doi.org/10.1155/2019/6131879

3. Bustin SA, Nolan T (2004) Pitfalls of quantitative real-time reverse transcription polymerase chain reaction. J Biomol Tech 15:155–166

4. Bustin SA, Benes V, Nolan T, Pfaffl MW (2005) Quantitative real-time RTPCR-a perspective. J Mol Endocrinol 34:597–601. https://doi.org/10.1677/jme.1.01755

5. Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, Zumla A (2005) The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem 344:141–143. https://doi.org/10.1016/j.ab.2005.05.022

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