Affiliation:
1. Endocrine Research Laboratories, King Edward Memorial Hospital for Women, Australia
Abstract
Abstract
The aim of this study was to find reasons for the interference of sex-hormone binding globulin (SHBG) in direct radioimmunoassays (RIAs) for testosterone and estradiol. 125I-labeled testosterone and estradiol were compared with 3H-labeled testosterone and estradiol for their affinity to SHBG. The 3H-labeled steroids bound far more avidly to SHBG than did the corresponding 125I-labeled steroids. The SHBG concentration in the standard matrix of two kits was significant. Finally, 3H-labeled steroids were compared with 125I-labeled steroids for their affinity to the antibody supplied with the kits. The 125I-labeled steroids bound more avidly than did the 3H-labeled steroids to the corresponding antibodies. Direct testosterone and estradiol results would be influenced according to the SHBG in the sample, as observed.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
29 articles.
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