Validation of Thin-Layer Chromatography Quantitation Determination with CCD Camera and Slit-Scanning Densitometer

Abstract

Abstract Video densitometric and classical slit-scanning determinations of pesticides on thin-layer chromatographic plates were validated for linearity, precision, and detection limit. A comparison of results showed that slit-scanning densitometry is more sensitive and more precise than video densitometry. However, according to validation requirements, relative standard deviation for video densitometry is acceptable (3.5–5.3%). Linearities are very good and almost identical for both techniques. The main advantage of video technology is speed (a few seconds, compared with 20 min with scanning densitometry). Video technology also provides excellent archiving facility. Image and chromatographic data can be stored together, edited, and used for many tasks.