Dry Rehydratable Film Method for Enumerating Confirmed Escherichia coli in Poultry, Meats, and Seafood: Collaborative Study
Author:
Gangar Vidhya1, Curiale Michael S1, Lindberg Kathryn2, Gambrel-Lenarz Sonya2, Adamson E, Barbari W, Brodsky M, Blackwell G, Bryce J, Cook P, Davis B, Deabel J, D’Onorio A, Follmi-Lieder E, Franklin J, Gambrel-Lenarz S, Horne S, James-Davis L, Lindgren S, McIntyre D, Moore J, Moorman M, Puccini M, Pulusani S, Sass A, Saunders L, Schop R, Sigua C A, Sinclair P, Story R, Westmoreland R, Windsor S, Witt J L,
Affiliation:
1. Silliker Laboratories Group, Inc., Corporate Research Center, 160 Armory Dr, South Holland, IL 60473 2. 3M Microbiology Products, 3M Center, Building 260-6B-01, St. Paul, MN 55144-1000
Abstract
Abstract
A rehydratable dry-film plating method for Escherichia coli, the Petrifilm E. coli/Coliform (EC) Count Plate in foods, has been compared with the AOAC INTERNATIONAL most probable number (MPN) method. Eleven laboratories participated in the collaborative study. Three E. coli levels in 8 samples each of frozen raw ground turkey, frozen raw ground beef, and frozen cooked fish were tested in duplicate. Mean log counts for the Petri film plate procedure were not significantly different from those for the MPN procedure for cooked fish samples inoculated with low or high inocula levels, for samples of raw turkey inoculated at medium level, and for beef inoculated at low, medium, and high levels. Repeatability and reproducibility vari ances of the Petrifilm EC Plate method recorded at 24 h were as good as or better than those of the MPN method. The dry rehydratable film method for enumerating confirmed E. coli in poultry, meats, and seafood has been adopted first action by AOAC INTERNATIONAL.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|