Introduction of a second “Green Revolution” mutation into wheat via in planta CRISPR/Cas9 delivery

Author:

Kumagai Yuya1,Liu Yuelin1,Hamada Haruyasu2,Luo Weifeng1ORCID,Zhu Jianghui1,Kuroki Misa1,Nagira Yozo2ORCID,Taoka Naoaki2,Katoh Etsuko3,Imai Ryozo1ORCID

Affiliation:

1. Genome-Edited Crop Development Group, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Kannondai 3-1-3, Tsukuba, Ibaraki 305-8604, Japan

2. Biotechnology Research Laboratories, Agri-Bio Research Center, Kaneka Corporation, Iwata, Shizuoka 438-0802, Japan

3. Structural Biology Team, Advanced Analysis Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8602, Japan

Abstract

Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction of a semidwarf1-orthologous mutation into an elite wheat variety.

Funder

JSPS

NARO Bio-oriented Technology Research Advancement Institution (BRAIN

Development of Innovative Technology

Cross-ministerial Strategic Innovation Promotion Program

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Reference15 articles.

1. Anther development : Basic principles and practical applications;Goldberg;Plant Cell,1993

2. An in planta biolistic method for stable wheat transformation;Hamada;Sci Rep,2017

3. Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat;Hamada;Sci Rep,2018

4. The genes of the Green Revolution;Hedden;Trends Genet,2003

5. In planta particle bombardment (iPB): A new method for plant transformation and genome editing;Imai;Plant Biotechnol,2020

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