Affiliation:
1. Department of Biological Sciences, University of Rhode Island, Kingston, Rhode Island 02881, USA
2. Wageningen UR Plant Breeding, Wageningen University and Research , Droevendaalsesteeg 1, 6708 PB Wageningen , The Netherlands
3. Department of Biochemistry and Molecular Biology, University of Nevada , Reno, Nevada 89557, USA
Abstract
Abstract
The common ancestor of seed plants and mosses contained homo-oligomeric cellulose synthesis complexes (CSCs) composed of identical subunits encoded by a single CELLULOSE SYNTHASE (CESA) gene. Seed plants use different CESA isoforms for primary and secondary cell wall deposition. Both primary and secondary CESAs form hetero-oligomeric CSCs that assemble and function in planta only when all the required isoforms are present. The moss Physcomitrium (Physcomitrella) patens has seven CESA genes that can be grouped into two functionally and phylogenetically distinct classes. Previously, we showed that PpCESA3 and/or PpCESA8 (class A) together with PpCESA6 and/or PpCESA7 (class B) form obligate hetero-oligomeric complexes required for normal secondary cell wall deposition. Here, we show that gametophore morphogenesis requires a member of class A, PpCESA5, and is sustained in the absence of other PpCESA isoforms. PpCESA5 also differs from the other class A PpCESAs as it is able to self-interact and does not co-immunoprecipitate with other PpCESA isoforms. These results are consistent with the hypothesis that homo-oligomeric CSCs containing only PpCESA5 subunits synthesize cellulose required for gametophore morphogenesis. Analysis of mutant phenotypes also revealed that, like secondary cell wall deposition, normal protonemal tip growth requires class B isoforms (PpCESA4 or PpCESA10), along with a class A partner (PpCESA3, PpCESA5, or PpCESA8). Thus, P. patens contains both homo-oligomeric and hetero-oligomeric CSCs.
Funder
National Science Foundation Awards
CRISPR-Cas9
University of Rhode Island Research Bridge Grant
Center for LignoCellulose Structure and Formation
Energy Frontier Research Center funded by the U.S. Department of Energy
Office of Basic Energy Sciences
DNA sequencing was conducted using the Rhode Island Genomics and Sequencing Center
Rhode Island NSF EPSCoR research facility
National Science Foundation EPSCoR Cooperative Agreement
Nevada Proteomics Center, which is supported by Nevada INBRE
National Institute of General Medical Sciences within the National Institutes of Health
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
Cited by
6 articles.
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