Interplay between NIN-LIKE PROTEINs 6 and 7 in nitrate signaling

Author:

Cheng Yu-Hsuan12ORCID,Durand Mickael3ORCID,Brehaut Virginie3ORCID,Hsu Fu-Chiun2ORCID,Kelemen Zsolt3ORCID,Texier Yves3ORCID,Krapp Anne3ORCID,Tsay Yi-Fang12ORCID

Affiliation:

1. Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and National Defense Medical Center , Taipei 114201 , Taiwan

2. Institute of Molecular Biology, Academia Sinica , Taipei 11529 , Taiwan

3. Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB) , Versailles 78000 , France

Abstract

Abstract NLP7 (NIN-LIKE-PROTEIN 7) is the major transcriptional factor responsible for the primary nitrate response (PNR), but the role of its homolog, NLP6, in nitrogen signaling and the interplay between NLP6 and NLP7 remain to be elucidated. In this study, we show that, like NLP7, nuclear localization of NLP6 via a nuclear retention mechanism is nitrate dependent, but nucleocytosolic shuttling of both NLP6 and NLP7 is independent of each other. Compared with single mutants, the nlp6nlp7 double mutant displays a synergistic growth retardation phenotype in response to nitrate. The transcriptome analysis of the PNR showed that NLP6 and NLP7 govern ∼50% of nitrate-induced genes, with cluster analysis highlighting 2 distinct patterns. In the A1 cluster, NLP7 plays the major role, whereas in the A2 cluster, NLP6 and NLP7 are partially functionally redundant. Interestingly, comparing the growth phenotype and PNR under high- and low-nitrate conditions demonstrated that NLP6 and NLP7 exert a more dominant role in the response to high nitrate. Apart from nitrate signaling, NLP6 and NLP7 also participated in high ammonium conditions. Growth phenotypes and transcriptome data revealed that NLP6 and NLP7 are completely functionally redundant and may act as repressors in response to ammonium. Other NLP family members also participated in the PNR, with NLP2 and NLP7 acting as broader regulators and NLP4, -5, -6, and -8 regulating PNR in a gene-dependent manner. Thus, our findings indicate that multiple modes of interplay exist between NLP6 and NLP7 that differ depending on nitrogen sources and gene clusters.

Funder

ANR IMANA

ANR NITRASENSE

Saclay Plant Sciences

National Science and Technology Council

Academia Sinica

Institute of Molecular Biology, Academia Sinica, Taiwan

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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