zma-miR159 targets ZmMYB74 and ZmMYB138 transcription factors to regulate grain size and weight in maize

Author:

Wang Qiyue12ORCID,Wan Jiong1,Dang Kuntai1,Meng Shujun1,Hu Desheng1,Lin Yuan12,Qiu Xiaoqian1,Guo Zhanyong1ORCID,Fu Zhiyuan1,Ding Dong1,Tang Jihua1

Affiliation:

1. National Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University , Zhengzhou, Henan 450002 , China

2. Maize Research Department, Hebi Academy of Agricultural Sciences , Hebi, Henan 458030 , China

Abstract

Abstract Endosperm cell number is critical in determining grain size in maize (Zea mays). Here, zma-miR159 overexpression led to enlarged grains in independent transgenic lines, suggesting that zma-miR159 contributes positively to maize grain size. Targeting of ZmMYB74 and ZmMYB138 transcription factor genes by zma-miR159 was validated using 5′ RACE and dual-luciferase assay. Lines in which ZmMYB74 was knocked out using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) presented a similar enlarged grain phenotype as those with zma-miR159 overexpression. Downstream genes regulating cell division were identified through DNA affinity purification sequencing using ZmMYB74 and ZmMYB138. Our results suggest that zma-miR159–ZmMYB modules act as an endosperm development hub, participating in the division and proliferation of endosperm cells.

Funder

National Natural Science Foundation of China

open funds of the State Key Laboratory of Crop Genetics and Germplasm Enhancement

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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