A CASE toolkit for easy and efficient multiplex transgene-free gene editing-Reference-Cited by-同舟云学术

A CASE toolkit for easy and efficient multiplex transgene-free gene editing

Published:2021-12-06 Issue: Volume: Page:
ISSN:1532-2548
Container-title:Plant Physiology
language:en
Short-container-title:

Author:

Liu Jin-Lei¹²,Chen Meng-Meng¹²,Chen Wen-Qiang¹²^ORCID,Liu Chun-Ming¹²³,He Yubing⁴⁵⁶^ORCID,Song Xiu-Fen¹²^ORCID

Affiliation:

1. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

3. School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China

4. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China

5. Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry, Nanjing Agricultural University, Nanjing 210095, China

6. Excellence and Innovation Center, Jiangsu Academy of Agricultural Science, Nanjing 210014, China

Abstract

An integrated transgene-free multiplex gene-editing toolkit based on the Transgene Killer CRISPR technology greatly saves labor, time, and cost.

Funder

National Natural Science Foundation of China

National Transgenic Science and Technology Program

Youth Innovation Promotion Association of the Chinese Academy of Sciences

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Link

https://academic.oup.com/plphys/advance-article-pdf/doi/10.1093/plphys/kiab573/41773047/kiab573.pdf

Reference20 articles.

1. Applications of multiplex genome editing in higher plants;Armario Najera;Curr Opin Biotechnol,2019

2. Generation of a multiplex mutagenesis population via pooled CRISPR-Cas9 in soya bean;Bai;Plant Biotechnol J,2020

3. A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants;Chen;Hortic Res,2018

4. Engineering introns to express RNA guides for Cas9- and Cpf1-mediated multiplex genome editing;Ding;Mol Plant,2018

5. An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing;Gao;Plant Physiol,2016

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