Novel CRISPR/Cas9 system assisted by fluorescence marker and pollen killer for high‐efficiency isolation of transgene‐free edited plants in rice
Author:
Affiliation:
1. State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences Changsha China
2. Longping Branch, College of Biology Hunan University Changsha China
Publisher
Wiley
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.14293
Reference6 articles.
1. Genome engineering for crop improvement and future agriculture
2. Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants
3. Cas9‐ PF , an early flowering and visual selection marker system, enhances the frequency of editing event occurrence and expedites the isolation of genome‐edited and transgene‐free plants
4. A CASE toolkit for easy and efficient multiplex transgene-free gene editing
5. Pollen self-elimination CRISPR–Cas genome editing prevents transgenic pollen dispersal in maize
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