Potassium transporter OsHAK18 mediates potassium and sodium circulation and sugar translocation in rice

Author:

Peng Lirun12ORCID,Xiao Huojun12ORCID,Li Ran12ORCID,Zeng Yang12ORCID,Gu Mian12ORCID,Moran Nava3ORCID,Yu Ling12ORCID,Xu Guohua12ORCID

Affiliation:

1. National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Ministry of Agriculture, Nanjing Agricultural University , Nanjing 210095 , China

2. Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University , Nanjing 210095 , China

3. The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem , Rehovot 76100 , Israel

Abstract

Abstract High-affinity potassium (K+) transporter (HAK)/K+ uptake permease (KUP)/K+ transporter (KT) have been identified in all genome-sequenced terrestrial plants. They play an important role in K+ acquisition and translocation and in enhancing salt tolerance. Here, we report that plasma membrane–located OsHAK18 functions in K+ and sodium (Na+) circulation and sugar translocation in rice (Oryza sativa). OsHAK18 was expressed mainly, though not exclusively, in vascular tissues and particularly in the phloem. Knockout (KO) of OsHAK18 reduced K+ concentration in phloem sap and roots but increased K+ accumulation in the shoot of both ‘Nipponbare’ and ‘Zhonghua11’ cultivars, while overexpression (OX) of OsHAK18 driven by its endogenous promoter increased K+ concentration in phloem sap and roots and promoted Na+ retrieval from the shoot to the root under salt stress. Split-root experimental analysis of rubidium (Rb+) uptake and circulation indicated that OsHAK18-OX promoted Rb+ translocation from the shoot to the root. In addition, OsHAK18-KO increased while OsHAK18-OX reduced soluble sugar content in the shoot and oppositely affected the sugar concentration in the phloem and its content in the root. Moreover, OsHAK18-OX dramatically increased grain yield and physiological K+ utilization efficiency. Our results suggest that—unlike other OsHAKs analyzed heretofore—OsHAK18 is critical for K+ and Na+ recirculation from the shoot to the root and enhances the source-to-sink translocation of photo-assimilates.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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