Tracing the transmission of mpox through wastewater surveillance in Southeast Asia

Author:

Wannigama Dhammika Leshan12345678910ORCID,Amarasiri Mohan11,Phattharapornjaroen Phatthranit121314,Hurst Cameron1516,Modchang Charin17181920ORCID,Chadsuthi Sudarat2122,Anupong Suparinthon1718,Miyanaga Kazuhiko2324,Cui Longzhu2324,Thuptimdang Wanwara2526,Ali Hosseini Rad S M2728,Fernandez Stefan2930,Huang Angkana T2930,Vatanaprasan Porames2324,Jay Dylan John910,Saethang Thammakorn3132,Luk-in Sirirat3334,Storer Robin James355,Ounjai Puey3637,Ragupathi Naveen Kumar Devanga678,Kanthawee Phitsanuruk3839,Sano Daisuke40414243,Furukawa Takashi11,Sei Kazunari11,Leelahavanichkul Asada234445,Kanjanabuch Talerngsak4647485495051,Higgins Paul G5253545556,Nanbo Asuka57,Kicic Anthony5859606162,Singer Andrew C63,Chatsuwan Tanittha2345,Trowsdale Sam64,Siow Richard656667666869,Shibuya Kenji70,Abe Shuichi1,Ishikawa Hitoshi71,Hongsing Parichart7273

Affiliation:

1. Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital , Aoyagi, Yamagata , Japan

2. Department of Microbiology , Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

3. Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society , Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

4. Center of Excellence in Antimicrobial Resistance and Stewardship , Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

5. Chulalongkorn University , Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

6. School of Medicine , Faculty of Health and Medical Sciences, , Nedlands, Western Australia , Australia

7. The University of Western Australia , Faculty of Health and Medical Sciences, , Nedlands, Western Australia , Australia

8. Biofilms and Antimicrobial Resistance Consortium of ODA receiving countries, The University of Sheffield , Sheffield, South Yorkshire , UK

9. Pathogen Hunter's Research Collaborative Team , Department of Infectious Diseases and Infection Control, , Aoyagi, Yamagata , Japan

10. Yamagata Prefectural Central Hospital , Department of Infectious Diseases and Infection Control, , Aoyagi, Yamagata , Japan

11. Laboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences/Graduate School of Medical Sciences, Kitasato University , Sagamihara-Minami, Kanagawa , Japan

12. Faculty of Medicine Ramathibodi Hospital, Mahidol University , Ratchathewi, Bangkok , Thailand

13. Institute of Clinical Sciences , Department of Surgery, Sahlgrenska Academy, , Universitetsplatsen 1, 405 30 Gothenburg , Sweden

14. Gothenburg University , Department of Surgery, Sahlgrenska Academy, , Universitetsplatsen 1, 405 30 Gothenburg , Sweden

15. Molly Wardaguga Research Centre, Charles Darwin University , Brisbane, Queensland , Australia

16. Statistics, QIMR Berghofer Medical Research Institute , Brisbane, Queensland , Australia

17. Biophysics Group , Department of Physics, Faculty of Science, , Bangkok , Thailand

18. Mahidol University , Department of Physics, Faculty of Science, , Bangkok , Thailand

19. Centre of Excellence in Mathematics, MHESI , Ratchathewi, Bangkok , Thailand

20. Thailand Center of Excellence in Physics, Ministry of Higher Education, Science, Research and Innovation , Ratchathewi, Bangkok , Thailand

21. Department of Physics , Faculty of Science, , Mueang Phitsanulok District, Phitsanulok , Thailand

22. Naresuan University , Faculty of Science, , Mueang Phitsanulok District, Phitsanulok , Thailand

23. Division of Bacteriology , School of Medicine, , Tochigi , Japan

24. Jichi Medical University , School of Medicine, , Tochigi , Japan

25. Institute of Biomedical Engineering , Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, , Hat Yai, Songkhla , Thailand

26. Prince of Songkla University , Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, , Hat Yai, Songkhla , Thailand

27. Department of Microbiology and Immunology, University of Otago , Dunedin, Otago , New Zealand

28. Center of Excellence in Immunology and Immune-Mediated Diseases, Chulalongkorn University , Pathum Wan Bangkok , Thailand

29. Department of Virology , U.S. Army Medical Directorate, , Ratchathewi, Bangkok , Thailand

30. Armed Forces Research Institute of Medical Sciences , U.S. Army Medical Directorate, , Ratchathewi, Bangkok , Thailand

31. Department of Computer Science , Faculty of Science, , Chatuchak, Bangkok , Thailand

32. Kasetsart University , Faculty of Science, , Chatuchak, Bangkok , Thailand

33. Department of Clinical Microbiology and Applied Technology , Faculty of Medical Technology, , Phutthamonthon District, Nakhon Pathom , Thailand

34. Mahidol University , Faculty of Medical Technology, , Phutthamonthon District, Nakhon Pathom , Thailand

35. Office of Research Affairs , Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

36. Department of Biology , Faculty of Science, , Ratchathewi, Bangkok , Thailand

37. Mahidol University , Faculty of Science, , Ratchathewi, Bangkok , Thailand

38. Public Health major , School of Health Science, , Mueang Chiang Rai District, Chiang Rai , Thailand

39. Mae Fah Luang University , School of Health Science, , Mueang Chiang Rai District, Chiang Rai , Thailand

40. Department of Frontier Sciences for Advanced Environment , Graduate School of Environmental Studies, , Sendai, Miyagi , Japan

41. Tohoku University , Graduate School of Environmental Studies, , Sendai, Miyagi , Japan

42. Department of Civil and Environmental Engineering , Graduate School of Engineering, , Sendai, Miyagi , Japan

43. Tohoku University , Graduate School of Engineering, , Sendai, Miyagi , Japan

44. Translational Research in Inflammation and Immunology Research Unit (TRIRU) , Department of Microbiology, , Pathum Wan, Bangkok , Thailand

45. Chulalongkorn University , Department of Microbiology, , Pathum Wan, Bangkok , Thailand

46. Division of Nephrology , Department of Medicine, Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

47. Chulalongkorn University , Department of Medicine, Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

48. Center of Excellence in Kidney Metabolic Disorders , Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

49. Dialysis Policy and Practice Program (DiP3) , School of Global Health, Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

50. Chulalongkorn University , School of Global Health, Faculty of Medicine, , Pathum Wan, Bangkok , Thailand

51. Peritoneal Dialysis Excellence Center, King Chulalongkorn Memorial Hospital , Pathum Wan, Bangkok , Thailand

52. Institute for Medical Microbiology , Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, , Albertus-Magnus-Platz, Cologne , Germany

53. University of Cologne , Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, , Albertus-Magnus-Platz, Cologne , Germany

54. German Centre for Infection Research, Partner site Bonn-Cologne , Albertus-Magnus-Platz, Cologne , Germany

55. Center for Molecular Medicine Cologne , Faculty of Medicine and University Hospital Cologne, , Albertus-Magnus-Platz, Cologne , Germany

56. University of Cologne , Faculty of Medicine and University Hospital Cologne, , Albertus-Magnus-Platz, Cologne , Germany

57. The National Research Center for the Control and Prevention of Infectious Diseases, Nagasaki University , Bunkyo-machi, Nagasaki , Japan

58. Wal-Yan Respiratory Research Centre , Telethon Kids Institute, , Nedlands, Western Australia , Australia

59. University of Western Australia , Telethon Kids Institute, , Nedlands, Western Australia , Australia

60. Centre for Cell Therapy and Regenerative Medicine, Medical School, The University of Western Australia , Nedlands, Western Australia , Australia

61. Department of Respiratory and Sleep Medicine, Perth Children’s Hospital , Nedlands, Western Australia , Australia

62. School of Population Health, Curtin University , Bentley, Bentley, 6102, Western Australia , Australia

63. UK Centre for Ecology & Hydrology , Crowmarsh Gifford, Wallingford , UK

64. School of Environmental Science, University of Auckland , Auckland CBD, Auckland , New Zealand

65. King's British Heart Foundation Centre of Research Excellence , School of Cardiovascular Medicine and Sciences, , Denmark Hill Campus, The James Black Centre, 125 Coldharbour Lane, London , UK

66. King's College London , School of Cardiovascular Medicine and Sciences, , Denmark Hill Campus, The James Black Centre, 125 Coldharbour Lane, London , UK

67. Vascular Biology and Inflammation Section , School of Cardiovascular Medicine and Sciences, , Denmark Hill Campus, The James Black Centre, 125 Coldharbour Lane, London , UK

68. Department of Physiology , Anatomy & Genetics, , Broad St, Oxford , UK

69. University of Oxford , Anatomy & Genetics, , Broad St, Oxford , UK

70. Tokyo Foundation for Policy Research , Tokyo , Japan

71. Yamagata Prefectural University of Health Sciences , Aoyagi, Yamagata , Japan

72. Mae Fah Luang University Hospital , Mueang Chiang Rai,Chiang Rai , Thailand

73. School of Integrative Medicine, Mae Fah Luang University , Mueang Chiang Rai,Chiang Rai , Thailand

Abstract

Abstract High population density and tourism in Southeast Asia increase the risk of mpox due to frequent interpersonal contacts. Our wastewater surveillance in six Southeast Asian countries revealed positive signals for Monkeypox virus (MPXV) DNA, indicating local transmission. This alerts clinicians and helps allocate resources like testing, vaccines and therapeutics in resource-limited countries.

Funder

Balvi Filantropic Fund and Chulalongkorn University

University of Western Australia

Yamagata Prefectural Central Hospital

Centre of Excellence in Mathematics

Ministry of Higher Education, Science, Research and Innovation, Thailand

Center of Excellence on Medical Biotechnology

Thailand Center of Excellence in Physics

Rothwell Family Fellow

Publisher

Oxford University Press (OUP)

Subject

General Medicine

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