Highly-automated, high-throughput replication of yeast-based logic circuit design assessments

Author:

Goldman Robert P1ORCID,Moseley Robert2,Roehner Nicholas3,Cummins Breschine4ORCID,Vrana Justin D5ORCID,Clowers Katie J6,Bryce Daniel1,Beal Jacob3ORCID,DeHaven Matthew1,Nowak Joshua7,Higa Trissha7,Biggers Vanessa7,Lee Peter6,Hunt Jeremy P7,Mosqueda Lorraine7,Haase Steven B2ORCID,Weston Mark8,Zheng George8,Deckard Anastasia9,Gopaulakrishnan Shweta10,Stubbs Joseph F10,Gaffney Niall I10,Vaughn Matthew W10,Maheshri Narendra6,Mikhalev Ekaterina6,Bartley Bryan3ORCID,Markeloff Richard3,Mitchell Tom3,Nguyen Tramy3,Sumorok Daniel3,Walczak Nicholas3,Myers Chris11,Zundel Zach12,Hatch Benjamin12,Scholz James12,Colonna-Romano John13

Affiliation:

1. SIFT, LLC , 319 First Ave, North, Suite 400, Minneapolis, MN 55401, USA

2. Department of Biology, Box 90338, Duke University , Durham, NC 27708, USA

3. BBN/Raytheon , 10 Moulton Street, Cambridge, MA 02138, USA

4. Department of Mathematical Sciences, Montana State University , P.O. Box 172400, Bozeman, MT 59717-2400, USA

5. Just – Evotec Biologics, 401 Terry Ave N, Seattle, WA 98109, USA

6. Ginkgo Bioworks, 27 Drydock Ave 8th Floor, Boston, MA 02210, USA

7. Strateos

8. Texas Advanced Computer Center (TACC), University of Texas , 10100 Burnet Rd, Austin, TX 78758, USA

9. Geometric Data Analytics, Inc., 343 W Main St, Durham, NC 27701, USA

10. Texas Advanced Computing Center, University of Texas at Austin

11. Department of Electrical, Computer & Energy Engineering, College of Engineering and Applied Science, University of Colorado , Boulder, 425 UCB #1B55, Boulder, CO 80309, USA

12. Department of Electrical and Computer Engineering, University of Utah , 50 Central Campus Dr #2110, Salt Lake City, UT 84112, USA

13. Aptima

Abstract

Abstract We describe an experimental campaign that replicated the performance assessment of logic gates engineered into cells of Saccharomyces cerevisiae by Gander et al. Our experimental campaign used a novel high-throughput experimentation framework developed under Defense Advanced Research Projects Agency’s Synergistic Discovery and Design program: a remote robotic lab at Strateos executed a parameterized experimental protocol. Using this protocol and robotic execution, we generated two orders of magnitude more flow cytometry data than the original experiments. We discuss our results, which largely, but not completely, agree with the original report and make some remarks about lessons learned. Graphical Abstract

Publisher

Oxford University Press (OUP)

Subject

Agricultural and Biological Sciences (miscellaneous),Biomedical Engineering,Biomaterials,Bioengineering,Biotechnology

Cited by 5 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3