Quantitative Determination of Chemical Constituents from Seeds of Nigella sativa L. Using HPLC-UV and Identification by LC-ESI-TOF

Abstract

Abstract An HPLC method was developed for the simultaneous determination of nine compounds of Nigella sativa L. The separation was achieved within 23 min by using C18 column material, a wateracetonitrile mobile phase, both containing 0.1 acetic acid gradient system and a temperature of 35C. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of nine compounds were in the range of 0.0910 and 0.325 g/mL, respectively. The wavelength used for quantification with the diode array detector was 205 and 260 nm. LC/MS coupled with electrospray ionization interface method is described for the identification of compounds in N. sativa L. samples. This method involved the use of [MH]<sup/> and [MNa]<sup/> ions in the positive ion mode with extracted ion chromatogram.