GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map-Reference-Cited by-同舟云学术

GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map

Published:2022-04-22 Issue:8 Volume:32 Page:646-650
ISSN:1460-2423
Container-title:Glycobiology
language:en
Short-container-title:

Author:

Yagi Hirokazu¹²^ORCID,Amagasa Eijiro³,Shiota Masaaki³,Yamada Issaku⁴^ORCID,Aoki-Kinoshita Kiyoko F³^ORCID,Kato Koichi¹²⁵^ORCID

Affiliation:

1. Graduate School of Pharmaceutical Sciences , Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603 , Japan

2. Exploratory Research Center on Life and Living Systems (ExCELLS) , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 , Japan

3. Glycan & Life Systems Integration Center (GaLSIC) , Soka University, 1-236 Tangi-machi, Hachioji, Tokyo 192-8577 , Japan

4. The Noguchi Institute , 1-9-7, Kaga, Itabashi-ku, Tokyo 173-0003 , Japan

5. Institute for Molecular Science , National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 , Japan

Abstract

Abstract High-performance liquid chromatography (HPLC) elution data provide a useful tool for quantitative glycosylation profiling, discriminating isomeric oligosaccharides. The web application Glycoanalysis by the Three Axes of MS and Chromatography (GALAXY), which is based on the three-dimensional HPLC map of N-linked oligosaccharides with pyridyl-2-amination developed by Dr. Noriko Takahashi, has been extensively used for N-glycosylation profiling at molecular, cellular, and tissue levels. Herein, we describe the updated GALAXY as version 3, which includes new HPLC data including those of glucuronylated and sulfated glycans, an improved graphical user interface using modern technologies, and linked to glycan information in GlyTouCan and the GlyCosmos Portal. This liaison will facilitate glycomic analyses of human and other organisms in conjunction with multiomics data.

Funder

National Bioscience Database Center

Publisher

Oxford University Press (OUP)

Subject

Biochemistry

Link

https://academic.oup.com/glycob/advance-article-pdf/doi/10.1093/glycob/cwac025/43835836/cwac025.pdf

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