Arabidopsis N6-methyladenosine methyltransferase FIONA1 regulates floral transition by affecting the splicing of FLC and the stability of floral activators SPL3 and SEP3

Author:

Cai Jing1,Hu Jianzhong1,Amara Umme1,Park Su Jung1,Li Yuxia2,Jeong Daesong3,Lee Ilha3ORCID,Xu Tao2ORCID,Kang Hunseung1ORCID

Affiliation:

1. Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University , Gwangju 61186 , Korea

2. Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University , Xuzhou, 221116, Jiangsu Province , China

3. School of Biological Sciences, Seoul National University , Seoul 08826 , Korea

Abstract

Abstract N 6-methyladenosine (m6A) RNA methylation has been shown to play a crucial role in plant development and floral transition. Two recent studies have identified FIONA1 as an m6A methyltransferase that regulates the floral transition in Arabidopsis through influencing the stability of CONSTANS (CO), SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), and FLOWERING LOCUS C (FLC). In this study, we confirmed that FIONA1 is an m6A methyltransferase that installs m6A marks in a small group of mRNAs. Furthermore, we show that, in addition to its role in influencing the stability of CO, SOC1, and FLC, FIONA1-mediated m6A methylation influences the splicing of FLC, a key floral repressor, and the stability of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 3 (SPL3) and SEPALLATA3 (SEP3), floral activators, which together play a vital role in floral transition in Arabidopsis. Our study confirms the function of FIONA1 as an m6A methyltransferase and suggests a close molecular link between FIONA1-mediated m6A methylation and the splicing of FLC and the destabilization of SPL3 and SEP3 in flowering time control.

Funder

National Research Foundation of Korea

Priority Academic Program Development of Jiangsu Higher Education Institutions

Ministry of Science, ICT and Future Planning

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

Reference68 articles.

1. HNRNPA2B1 is a mediator of m6A-dependent nuclear RNA processing events;Alarcón;Cell,2015

2. Mechanistic insights into m6A modification of U6 snRNA by human METTL16;Aoyama;Nucleic Acids Research,2020

3. Occurrence and functions of m6A and other covalent modifications in plant mRNA;Arribas-Hernández;Plant Physiology,2020

4. An m6A-YTH module controls developmental timing and morphogenesis in Arabidopsis;Arribas-Hernández;The Plant Cell,2018

5. MODOMICS: a database of RNA modification pathways. 2017 update;Boccaletto;Nucleic Acids Research,2018

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3