In vitro activity of hypochlorous acid generating electrochemical bandage against monospecies and dual-species bacterial biofilms

Author:

Kletzer Joseph12,Raval Yash S2,Mohamed Abdelrhman3ORCID,Mandrekar Jayawant N4,Greenwood-Quaintance Kerryl E2,Beyenal Haluk3,Patel Robin25ORCID

Affiliation:

1. Paracelsus Medical University , Salzburg 5020 , Austria

2. Division of Clinical Microbiology, Mayo Clinic Rochester , Rochester, MN 55905 , United States

3. The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University , Pullman, WA 99164 , United States

4. Department of Quantitative Health Sciences, Mayo Clinic , Rochester, MN 55905 , United States

5. Division of Public Health, Infectious Diseases, and Occupational Medicine, Mayo Clinic , Rochester, MN 55905 , United States

Abstract

Abstract Aims As antimicrobial resistance is on the rise, treating chronic wound infections is becoming more complex. The presence of biofilms in wound beds contributes to this challenge. Here, the activity of a novel hypochlorous acid (HOCl) producing electrochemical bandage (e-bandage) against monospecies and dual-species bacterial biofilms formed by bacteria commonly found in wound infections was assessed. Methods and results The system was controlled by a wearable potentiostat powered by a 3V lithium-ion battery and maintaining a constant voltage of + 1.5V Ag/AgCl, allowing continuous generation of HOCl. A total of 19 monospecies and 10 dual-species bacterial biofilms grown on polycarbonate membranes placed on tryptic soy agar (TSA) plates were used as wound biofilm models, with HOCl producing e-bandages placed over the biofilms. Viable cell counts were quantified after e-bandages were continuously polarized for 2, 4, 6, and 12 hours. Time-dependent reductions in colony forming units (CFUs) were observed for all studied isolates. After 12 hours, average CFU reductions of 7.75 ± 1.37 and 7.74 ± 0.60 log10 CFU/cm2 were observed for monospecies and dual-species biofilms, respectively. Conclusions HOCl producing e-bandages reduce viable cell counts of in vitro monospecies and dual-species bacterial biofilms in a time-dependent manner in vitro. After 12 hours, >99.999% reduction in cell viability was observed for both monospecies and dual-species biofilms.

Funder

National Institute of Allergy and Infectious Diseases

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

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