DUAL ACTION ELECTROCHEMICAL BANDAGE OPERATED by a PROGRAMMABLE MULTIMODAL WEARABLE POTENTIOSTAT

Abstract

ABSTRACTElectrochemical bandages (e-bandages) can be applied to biofilm-infected wounds to generate reactive oxygen species, such as hypochlorous acid (HOCl) or hydrogen peroxide (H2O2). The e-bandage-generated HOCl or H2O2kills biofilmsin vitroand in infected wounds on mice. The HOCl-generating e-bandage is more active against biofilmsin vitro, although this distinction is less apparentin vivo. The H2O2-generating e-bandage, more than the HOCl-generating e-bandage, is associated with improved healing of infected wounds. A strategy in which H2O2and HOCl are generated alternately—for dual action—was explored. The goal was to develop a programmable multimodal wearable potentiostat (PMWP) that could be programmed to generate HOCl or H2O2, as needed. An ultralow-power microcontroller unit managed operation of the PMWP. The system was operated with a 260-mAh capacity coin battery and weighed 4.6 grams, making it suitable for small animal experiments or human use. The overall cost of a single wearable potentiostat was $6.50 (USD). The device was verified using established electrochemical systems and functioned comparably to a commercial potentiostat. To determine antimicrobial effectiveness, PMWP-controlled e-bandages were tested against clinical isolates of four prevalent chronic wound bacterial pathogens, methicillin-resistantStaphylococcus aureus(MRSA),Pseudomonas aeruginosa, Acinetobacter baumannii, andEnterococcus faecium, and one fungal pathogen of emerging concern,Candida auris. PMWP-controlled e-bandages exhibited broad-spectrum activity against biofilms of all study isolates tested when programmed to deliver HOCl followed by H2O2. These results show that the PMWP operates effectively and is suitable for animal testing.