Standardisation of the in vitro comet assay: influence of lysis time and lysis solution composition on the detection of DNA damage induced by X-rays-Reference-Cited by-同舟云学术

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Standardisation of the in vitro comet assay: influence of lysis time and lysis solution composition on the detection of DNA damage induced by X-rays

Published:2018-01 Issue:1 Volume:33 Page:25-30
ISSN:0267-8357
Container-title:Mutagenesis
language:en
Short-container-title:

Author:

Enciso José M¹,Gutzkow Kristine B²³,Brunborg Gunnar²³,Olsen Ann-Karin²³,López de Cerain Adela¹,Azqueta Amaya¹

Affiliation:

1. Department of Pharmacology and Toxicology, University of Navarra, and IdiSNA, Navarra Institute for Health Research, Pamplona, Spain

2. Department of Molecular Biology, Norwegian Institute of Public Health, Oslo, Norway

3. Centre for Environmental Radioactivity (CoE CERAD), Norway

Funder

Ministry of Economy, Industry and Competitiveness

Research Council of Norway

Publisher

Oxford University Press (OUP)

Subject

Health, Toxicology and Mutagenesis,Genetics (clinical),Toxicology,Genetics

Link

http://academic.oup.com/mutage/article-pdf/33/1/25/24161539/gex039.pdf

Reference38 articles.

1. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells;Ostling;Biochem. Biophys. Res. Commun,1984

2. A simple technique for quantitation of low levels of DNA damage in individual cells;Singh;Exp. Cell Res,1988

3. The essential comet assay: a comprehensive guide to measuring DNA damage and repair;Azqueta;Arch. Toxicol,2013

4. Enhancing the sensitivity of the comet assay as a genotoxicity test, by combining it with bacterial repair enzyme FPG;Azqueta;Mutagenesis,2013

5. The use of bacterial repair endonucleases in the comet assay;Collins;Methods Mol. Biol,2017

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1. May DNA analyses be biased by hidden oxidative damage? Voltammetric study of temperature and oxidation stress effect;PLOS ONE;2024-06-14

2. Reducing risk of false positives in the in vivo comet assay and improving result reliability;Mutation Research - Genetic Toxicology and Environmental Mutagenesis;2024-04

3. Further development of CometChip technology to measure DNA damage in vitro and in vivo: Comparison with the 2 gels/slide format of the standard and enzyme-modified comet assay;Toxicology;2024-01

4. Four Decades of the Comet Assay: pH Optimum of Lysis Buffer Still Needs to be Elucidated;Anti-Cancer Agents in Medicinal Chemistry;2023-10

5. Inter-laboratory variation in measurement of DNA damage by the alkaline comet assay in the hCOMET ring trial;Mutagenesis;2023-05-25

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