A DNA Extraction Method for Insects From Sticky Traps: Targeting a Low Abundance Pest, Phthorimaea absoluta (Lepidoptera: Gelechiidae), in Mixed Species Communities

Author:

Butterwort V1ORCID,Dansby H1,Zink F A2ORCID,Tembrock L R2ORCID,Gilligan T M3ORCID,Godoy A4ORCID,Braswell W E4ORCID,Kawahara A Y1ORCID

Affiliation:

1. McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida , Gainesville, FL 32511 , USA

2. Department of Agricultural Biology, 1177 Campus Delivery, Colorado State University , Fort Collins, CO 80523 , USA

3. USDA-APHIS-PPQ-Science & Technology, Identification Technology Program , 2301 Research Boulevard, Suite 108, Fort Collins, CO 80526 , USA

4. USDA-APHIS-PPQ-Science & Technology, Insect Management and Molecular Diagnostics Laboratory , 22675 N. Moorfield Road, Building 6414, Edinburg, TX 78541 , USA

Abstract

Abstract Invasive insects can cause catastrophic damage to ecosystems and cost billions of dollars each year due to management expenses and lost revenue. Rapid detection is an important step to prevent invasive insects from spreading, but improvements in detection capabilities are needed for bulk collections like those from sticky traps. Here we present a bulk DNA extraction method designed for the detection of Phthorimaea absoluta Meyrick (Lepidoptera: Gelechiidae), an invasive moth that can decimate tomato crops. We test the extraction method for insect specimens on sticky traps, subjected to different temperature and humidity conditions, and among mock insect communities left in the field for up to 21 d. We find that the extraction method yielded high success (>92%) in recovering target DNA across field and lab trials, without a decline in recovery after three weeks, across all treatments. These results may have a large impact on tomato growing regions where P. absoluta is in the early stages of invasion or not yet present. The extraction method can also be used to improve detection capabilities for other bulk insect collections, especially those using sticky traps, to the benefit of pest surveys and biodiversity studies.

Funder

United States Department of Agriculture’s Animal and Plant Health Inspection Service (APHIS) Farm Bill Section 10007

Publisher

Oxford University Press (OUP)

Subject

Insect Science,Ecology,General Medicine

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