Development of an on‐site diagnostic <scp>LAMP</scp> assay for rapid differentiation of the invasive pest <scp><i>Phthorimaea absoluta</i></scp> (Meyrick) using insect tissues-Reference-Cited by-同舟云学术

Development of an on‐site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues

Published:2024-04-10 Issue:8 Volume:80 Page:4069-4073
ISSN:1526-498X
Container-title:Pest Management Science
language:en
Short-container-title:Pest Management Science

Author:

Yang Li‐Feng¹²^ORCID,Liu Ya‐Ge¹²,Tao Yun‐Li¹²,Zhang Wan‐Min³,Li Jian‐Yong⁴,Chi Sheng‐Qi¹²,Zhang Gui‐Fen⁵^ORCID,Chu Dong¹²

Affiliation:

1. Shandong Engineering Research Center for Environment‐friendly Agricultural Pest Management, College of Plant Health and Medicine Qingdao Agricultural University Qingdao China

2. Shandong Province Center for Bioinvasions and Eco‐security Qingdao Agricultural University Qingdao China

3. Department of Pest Prediction and Management Liaoning Technological Center of Green Agriculture Shenyang China

4. Tech Center of Jinan Customs District Jinan China

5. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Integrated Pest Management of Crop, Ministry of Agriculture and Rural Affairs Institute of Plant Protection, Chinese Academy of Agricultural Sciences Beijing China

Abstract

AbstractBACKGROUNDThe tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on‐site identification of P. absoluta is urgently needed for interception of this pest across China.RESULTSWe developed a loop‐mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on‐site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment.CONCLUSIONIn the present research, we developed an on‐site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China. © 2024 Society of Chemical Industry.

Funder

National Key Research and Development Program of China

Taishan Scholar Foundation of Shandong Province

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/ps.8114

Reference32 articles.

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