Two-Day Hydrophobic Grid Membrane Filter Method for Yeast and Mold Enumeration in Foods Using YM-11 Agar: Collaborative Study
Author:
Entis Phyllis1, Athar A, Ballenger M, Bendeck M S, Birbari W, Brock G, Curiale M S, Estvander J, Fung D Y C, Green K, Ingham S C, Jafary M M, Jagow J A, Kalinowski R, Kelley G, Lee Y J, Lerner I, Lin C C S, Mendenhall C K, Tomer J, Reyes D, Rivera G, Rudolph C, Ryu J-H, Sado P, Snider J, Soto-Lopez L, Thakur R A H, Watson J, White S A, Wilkin E, Williams W D,
Affiliation:
1. QA Life Sciences, Inc., 6645 Nancy Ridge Dr, San Diego, CA 92121
Abstract
Abstract
Twenty laboratories participated in a collaborative study to validate a 2-day hydrophobic grid membrane filter method using YM-11 agar for enumeration of yeast and mold in foods. Six naturally contaminated food products were included in the study: garlic powder, raw ground beef, walnuts, flour/meal, orange juice, and yogurt. The test method produced significantly higher results than the 5-day pour plate reference method for orange juice and significantly lower, though numerically similar, results for walnuts and yogurt. Differences between the test and reference methods were not significant for garlic powder, raw ground beef, or flour/meal. Repeatability and reproducibility were similar for both the test and reference methods in all cases. The hydrophobic grid membrane filter method for enumeration of yeast and mold in foods has been adopted by AOAC INTERNATIONAL.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
6 articles.
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