Affiliation:
1. Universitat Jaume I, Qumica Bioanaltica, Q.F.A., E.S.T.C.E., 12071 Castell, Spain
Abstract
Abstract
A simple and reliable HPLC method was developed to determine imidacloprid, a chloronicotinyl insecticide that has a highly specific affinity to the nicotinic acetylcholine receptor of insects, above its permissible limit of consumption in fruit juices (orange, apple, and a mixture of pineapple and pear). Samples were injected directly into a Kromasil C18 column, without any pretreatment step, using the micellar mobile phase 0.10 M sodium dodecyl sulfate and 2.5 (v/v) propanol buffered at pH 7 and UV detection at 210 nm. Under these conditions, imidacloprid was eluted in <4 min with no interference by the endogenous compounds of the juice fruits. The analytical parameters linearity (r > 0.9998) and intraday and interday precision (RSD 0.1 2.8 and 0.077, respectively) were obtained in the method validation. LOD and LOQ were calculated to be 0.4 and 1.5 ng/mL, respectively. Recoveries were in the 98103 range. The simplicity of the method makes it a good candidate for application in routine analysis in the area of food control and quality evaluation.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
9 articles.
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