Interferon-γ Release Assay for Accurate Detection of Severe Acute Respiratory Syndrome Coronavirus 2 T-Cell Response

Author:

Murugesan Kanagavel1,Jagannathan Prasanna2,Pham Tho D13,Pandey Suchitra13,Bonilla Hector F2,Jacobson Karen2,Parsonnet Julie2,Andrews Jason R2,Weiskopf Daniela4,Sette Alessandro45,Pinsky Benjamin A126,Singh Upinder2,Banaei Niaz127

Affiliation:

1. Department of Pathology, Stanford University School of Medicine, Stanford, California, USA

2. Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA

3. Stanford Blood Center, Stanford Health Care, Stanford, California, USA

4. Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, California, USA

5. Department of Medicine, Division of Infectious Diseases and Global Public Health, University of California, San Diego, La Jolla, California, USA

6. Clinical Virology Laboratory, Stanford Health Care, Stanford, California, USA

7. Clinical Microbiology Laboratory, Stanford Health Care, Stanford, California, USA

Abstract

Abstract We investigated feasibility and accuracy of an interferon-γ release assay (IGRA) for detection of T-cell responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Whole blood IGRA accurately distinguished between convalescent and uninfected healthy blood donors with a predominantly CD4+ T-cell response. SARS-CoV-2 IGRA may serve as a useful diagnostic tool in managing the coronavirus disease 2019 pandemic.

Funder

National Institutes of Health

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Microbiology (medical)

Reference11 articles.

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3. Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans;Mateus;Science,2020

4. Robust T cell immunity in convalescent individuals with asymptomatic or mild COVID-19;Sekine;Cell,2020

5. A sequence homology and bioinformatic approach can predict candidate targets for immune responses to SARS-CoV-2;Grifoni;Cell Host Microbe,2020

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