THE GENETIC SYSTEM CONTROLLING HOMOTHALLISM IN SACCHAROMYCES YEASTS

Abstract

ABSTRACT There are four types of life cycles in Saccharomyces cerevisiae and its related species. A perfect homothallic life cycle (the Ho type) is observed in the classic D strain. Two other types show semi-homothallism; one of them shows a 2-homothallic diploid:2α heterothallic haploid segregation (the Hp type) and another, a 2-homothallic:2a segregation (the Hq type). In the segregants from these Ho, Hp, and Hq diploids, each homothallic segregant shows the same segregation pattern as its parental diploid. The fourth type has a heterothallic life cycle showing a 2a:2α segregation and the diploids are produced by the fusion of two haploid cells of opposite mating types. The diploids prepared by the crosses of α Hp (an α haploid segregant from the Hp diploid) to a Hq (an a haploid from the Hq diploid) segregated two types (Type I and II) of the Ho type homothallic clone among their meiotic segregants. Genetic analyses were performed to investigate this phenomenon and the genotypes of the Ho type homothallic clones of Type I and Type II. Results of these genetic analyses have been most adequately explained by postulating three kinds of homothallic genes, each consisting of a single pair of alleles, HO/ho, HMα/hmα, and HMa/hma, respectively. One of them, the HMα locus, was proved to be loosely linked (64 stranes) to the mating-type locus. A spore having the HO hmα hma genotype gives rise to an Ho type homothallic diploid (Type I), the same as in the case of the D strain which has the HO HMα HMa genotype (Type II). A spore having the a HO hmα HMa or α HO HMα hma genotype will produce an Hp or Hq type homothallic diploid culture, respectively. The other genotypes, a HO HMα hma, α HO hmα HMa, and the genotypes combined with the ho allele give a heterothallic character to the spore culture. A possible molecular hypothesis for the mating-type differentiation with the controlling elements produced by the HMα and HMa genes is proposed.