Determination of Histamine in Tomatoes by Capillary Electrophoresis

Author:

Bolygo Elek1,Cooper Paul A1,Jessop K Michael1,Moffatt Frank1

Affiliation:

1. Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire RG42 6ET, UK

Abstract

Abstract An improved capillary electrophoresis assay for histamine in crude extracts was developed and used to determine histamine levels in a selection of tomato fruits and pastes. Performance in terms of reproducibility and sensitivity was optimized by use of a high sensitivity detector flow cell, sodium hydroxide rinses, and a voltage gradient. The method was linear down to 0.2 μg/mL (signal-to-noise ratio = 4:1), which was below the endogenous level in all samples.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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