The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a −70/+96 mRNA region with respect to the translation initiation codon-Reference-Cited by-同舟云学术

The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a −70/+96 mRNA region with respect to the translation initiation codon

Published:2007-12-15 Issue:1 Volume:36 Page:e6-e6
ISSN:1362-4962
Container-title:Nucleic Acids Research
language:en
Short-container-title:

Author:

Care S.,Bignon C.,Pelissier M. C.,Blanc E.,Canard B.,Coutard B.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

http://academic.oup.com/nar/article-pdf/36/1/e6/16747292/gkm1097.pdf

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4. Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis;de Smit;Proc. Natl Acad. Sci. USA,2003

5. Rapid and easy thermodynamic optimization of the 5'-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli;Cebe;Protein Expr. Purif.,2006

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