Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR
Author:
Publisher
Elsevier BV
Subject
Biotechnology
Reference32 articles.
1. Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity;Skerra;Nucleic Acids Res.,1992
2. Effective amplification of long targets from cloned inserts and human genomic DNA;Cheng;Proc. Natl. Acad. Sci. USA,1994
3. Low incorporation of dUMP by some thermostable DNA polymerases may limit their use in PCR amplifications;Slupphaug;Anal. Biochem.,1993
4. Archaebacterial DNA polymerases tightly bind uracil containing DNA;Lasken;J. Biol. Chem.,1996
5. Structural basis for uracil recognition by archaeal family B DNA polymerases;Fogg;Nat. Struct. Biol.,2002
Cited by 20 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches;Bioengineering;2023-09-30
2. Expression and physicochemical characterization of an N-terminal polyhistidine-tagged phosphotriesterase from the soil bacterium Brevundimonas diminuta;Biocatalysis and Agricultural Biotechnology;2020-10
3. Heterologous expression of Oenococcus oeni sHSP20 confers temperature stress tolerance in Escherichia coli;Cell Stress and Chaperones;2018-01-22
4. Characterization and functional studies of fowl adenovirus 9 dUTPase;Virology;2016-10
5. Molecular characterization of Plasmodium falciparum uracil-DNA glycosylase and its potential as a new anti-malarial drug target;Malaria Journal;2014-04-17
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3