Genealogical Analyses of 3 Cultivated and 1 Wild Specimen of Vitis vinifera from Greece

Abstract

Abstract Grapevine (Vitis vinifera) has been an important crop with considerable cultural and economic significance for over 2,500 years, and Greece has been an important entry point into Europe for lineages that were domesticated in Western Asia and the Caucasus. However, whole-genome-based investigation of the demographic history of Greek cultivars relative to other European lineages has only started recently. To understand how Greek cultivars relate to Eurasian domesticated and wild populations, we sequenced 3 iconic domesticated strains (‘Xinomavro,’ ‘Agiorgitiko,’ ‘Mavrotragano’) along with 1 wild accession (the vinetree of Pausanias—a historically important wild specimen) and analyzed their genomic diversity together with a large sample of publicly available domesticated and wild strains. We also reconstructed genealogies by leveraging the powerful tsinfer methodology which has not previously been used in this system. We show that cultivated strains from Greece differ genetically from other strains in Europe. Interestingly, all the 3 cultivated Greek strains clustered with cultivated and wild accessions from Transcaucasia, South Asia, and the Levant and are amongst the very few cultivated European strains belonging to this cluster. Furthermore, our results indicate that ‘Xinomavro’ shares close genealogical proximity with European elite cultivars such as ‘Chardonnay,’ ‘Riesling,’ and ‘Gamay’ but not ‘Pinot.’ Therefore, the proximity of ‘Xinomavro’ to Gouais/Heunisch Weiss is confirmed and the utility of ancestral recombination graph reconstruction approaches to study genealogical relationships in crops is highlighted.