New dot-blot method for evaluating the effect of inactivators on mite and Japanese cedar pollen allergens

Author:

Yoshida Megumi12,Mizukami Keijiro1,Kurata Keigo2,Nasukawa Tadahiro1,Uchiyama Jumpei1,Sakaguchi Masahiro12ORCID

Affiliation:

1. Department of Veterinary Microbiology I, School of Veterinary Medicine, Azabu University, Sagamihara, Japan

2. ITEA Inc., Institute of Tokyo Environmental Allergy, Tokyo, Japan

Abstract

ABSTRACT As a method of evaluating the effect of inactivators on allergens while suppressing the effect of inactivator on the assay, we developed new dot-blot method that combines immunostaining and protein detection methods. This method is useful for evaluating whether the inactivator can inactivate allergens rather than removing them from the assay.

Funder

ITEA

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Reference6 articles.

1. Reduction of antigenicity of Cry j 1, a major allergen of Japanese cedar pollen, by thermal denaturation;Aoki;J Agric Food Chem,2009

2. Immunoassays for indoor allergens;Chapman;Clin Rev Allergy Immunol,2000

3. Effects of boiling on the IgE-binding properties of tropomyosin of shrimp (Litopenaeus vannamei);Liu;J Food Sci,2010

4. Conformational stability of B cell epitopes on group I and group II Dermatophagoides spp. allergens. Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies;Lombardero;J Immunol,1990

5. Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis;Sakaguchi;Immunology,1997

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3