A SlMYB75-centred transcriptional cascade regulates trichome formation and sesquiterpene accumulation in tomato

Author:

Gong Zehao1ORCID,Luo Yingqing1,Zhang Wenfa1ORCID,Jian Wei1ORCID,Zhang Lu2ORCID,Gao Xueli1ORCID,Hu Xiaowei1ORCID,Yuan Yujin1ORCID,Wu Mengbo1,Xu Xin1ORCID,Zheng Xianzhe1ORCID,Wu Guanle1,Li Zhengguo1ORCID,Li Zhi3ORCID,Deng Wei1ORCID

Affiliation:

1. Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing, China

2. Department of Horticulture and Landscape Architecture, Oklahoma State University, Stillwater, OK, USA

3. Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China

Abstract

Abstract Tomato trichomes act as a mechanical and chemical barrier against pests. An R2R3 MYB transcription factor gene, SlMYB75, is highly expressed in type II, V, and VI trichomes. SlMYB75 protein is located in the nucleus and possesses transcriptional activation activity. Down-regulation of SlMYB75 increased the formation of type II, V, and VI trichomes, accumulation of δ-elemene, β-caryophyllene, and α-humulene in glandular trichomes, and tolerance to spider mites in tomato. In contrast, overexpression of SlMYB75 inhibited trichome formation and sesquiterpene accumulation, and increased plant sensitivity to spider mites. RNA-Seq analyses of the SlMYB75 RNAi line indicated massive perturbation of the transcriptome, with a significant impact on several classes of transcription factors. Expression of the MYB genes SlMYB52 and SlTHM1 was strongly reduced in the RNAi line and increased in the SlMYB75-overexpressing line. SlMYB75 protein interacted with SlMYB52 and SlTHM1 and activated their expression. SlMYB75 directly targeted the promoter of the cyclin gene SlCycB2, increasing its activity. The auxin response factor SlARF4 directly targeted the promoter of SlMYB75 and inhibited its expression. SlMYB75 also bound to the promoters of the terpene synthase genes SlTPS12, SlTPS31, and SlTPS35, inhibiting their transcription. Our findings indicate that SlMYB75 perturbation affects several transcriptional circuits, resulting in altered trichome density and metabolic content.

Funder

National Key R&D Program of China

Project of Chongqing Science and Technology Commission

National Natural Science Foundation of China

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

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