Affiliation:
1. College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China
Abstract
Abstract
ABA-INSENSITIVE 3 (ABI3) has long been known for activation of storage protein accumulation. A role of ABI3 on oil accumulation was previously suggested based on a decrease of oil content in seeds of abi3 mutant. However, this conclusion could not exclude possibilities of indirect or pleiotropic effects, such as through mutual regulatory interactions with FUSCA3 (FUS3), an activator of oil accumulation. To identify that ABI3 functions independent of the effects of related seed transcription factors, we expressed ABI3 under the control of an inducible promoter in tobacco BY2 cells and Arabidopsis rosette leaves. Inducible expression of ABI3 activated oil accumulation in these non-seed cells, demonstrating a general role of ABI3 in regulation of oil biosynthesis. Further expressing ABI3 in rosette leaves of fus3 knockout mutant still caused up to 3-fold greater triacylglycerol accumulation, indicating ABI3 can activate lipid accumulation independently of FUS3. Transcriptome analysis revealed that LIPID DROPLET PROTEIN (LDP) genes, including OLEOSINs and CALEOSINs, were up-regulated up to 1000-fold by ABI3 in the absence of FUS3, while the expression of WRINKLED1 was doubled. Taken together, our results provide genetic evidence that ABI3 activates oil accumulation with or without FUS3, most likely through up-regulating LDPs and WRINKLED1.
Funder
National Natural Science Foundation of China
Yang Ling Seed Industry Innovation Center
Programme of Introducing Talents of Innovative Discipline to Universities
State Administration of Foreign Experts Affairs
Publisher
Oxford University Press (OUP)
Reference94 articles.
1. Genomic analysis and expression investigation of steroleosin gene family in Arabidopsis thaliana.;Aziz;Journal of Animal and Plant Sciences,2020
2. Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by master transcriptional regulators in Arabidopsis seed.;Baud;Plant Physiology,2016
3. WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis.;Baud;The Plant Journal,2007
4. Importance of the B2 domain of the Arabidopsis ABI3 protein for Em and 2S albumin gene regulation.;Bies-Etheve;Plant Molecular Biology,1999
5. The ABSCISIC ACID INSENSITIVE 3 (ABI3) gene is modulated by farnesylation and is involved in auxin signaling and lateral root development in Arabidopsis.;Brady;The Plant Journal,2003
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