Proteome plasticity during Physcomitrium patens spore germination – from the desiccated phase to heterotrophic growth and reconstitution of photoautotrophy

Author:

Hembach Lea1,Niemeyer Philipp W.2,Schmitt Kerstin3,Zegers Jaccoline M. S.4,Scholz Patricia5,Brandt Dennis6,Dabisch Janis J.1,Valerius Oliver3,Braus Gerhard H.3,Schwarzländer Markus6ORCID,de Vries Jan4ORCID,Rensing Stefan A.78ORCID,Ischebeck Till12ORCID

Affiliation:

1. Green Biotechnology, Institute of Plant Biology and Biotechnology (IBBP) University of Münster 48143 Münster Germany

2. Department of Plant Biochemistry, Albrecht‐von‐Haller‐Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB) University of Göttingen 37077 Göttingen Germany

3. Department for Molecular Microbiology and Genetics, Genetics and Göttingen Center for Molecular Biosciences (GZMB) and Service Unit LCMS Protein Analytics, Institute for Microbiology University of Göttingen 37077 Göttingen Germany

4. Department of Applied Bioinformatics, Göttingen Center for Molecular Biosciences (GZMB) and Campus Institute Data Science (CIDAS), Institute for Microbiology and Genetics University of Göttingen 37077 Göttingen Germany

5. Laboratoire Reproduction et Développement des Plantes (RDP), UCB Lyon 1, CNRS, INRAE Université de Lyon, ENS de Lyon Lyon France

6. Plant Energy Biology, Institute of Plant Biology and Biotechnology (IBBP) University of Münster 48143 Münster Germany

7. Plant Cell Biology, Department of Biology University of Marburg Marburg Germany

8. BIOSS Centre for Biological Signalling Studies University of Freiburg Freiburg Germany

Abstract

SUMMARYThe establishment of moss spores is considered a milestone in plant evolution. They harbor protein networks underpinning desiccation tolerance and accumulation of storage compounds that can be found already in algae and that are also utilized in seeds and pollen. Furthermore, germinating spores must produce proteins that drive the transition through heterotrophic growth to the autotrophic plant. To get insight into the plasticity of this proteome, we investigated it at five timepoints of moss (Physcomitrium patens) spore germination and in protonemata and gametophores. The comparison to previously published Arabidopsis proteome data of seedling establishment showed that not only the proteomes of spores and seeds are functionally related, but also the proteomes of germinating spores and young seedlings. We observed similarities with regard to desiccation tolerance, lipid droplet proteome composition, control of dormancy, and β‐oxidation and the glyoxylate cycle. However, there were also striking differences. For example, spores lacked any obvious storage proteins. Furthermore, we did not detect homologs to the main triacylglycerol lipase in Arabidopsis seeds, SUGAR DEPENDENT1. Instead, we discovered a triacylglycerol lipase of the oil body lipase family and a lipoxygenase as being the overall most abundant proteins in spores. This finding indicates an alternative pathway for triacylglycerol degradation via oxylipin intermediates in the moss. The comparison of spores to Nicotiana tabacum pollen indicated similarities for example in regards to resistance to desiccation and hypoxia, but the overall developmental pattern did not align as in the case of seedling establishment and spore germination.

Funder

Deutsche Forschungsgemeinschaft

European Research Council

Publisher

Wiley

Subject

Cell Biology,Plant Science,Genetics

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