Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function-Reference-Cited by-同舟云学术

Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function

Published:2020-05-04 Issue:1 Volume:117 Page:43-59
ISSN:0008-6363
Container-title:Cardiovascular Research
language:en
Short-container-title:

Author:

Zacchigna Serena¹²^ORCID,Paldino Alessia¹,Falcão-Pires Inês³,Daskalopoulos Evangelos P⁴,Dal Ferro Matteo¹^ORCID,Vodret Simone²^ORCID,Lesizza Pierluigi¹^ORCID,Cannatà Antonio¹,Miranda-Silva Daniela³,Lourenço André P³,Pinamonti Bruno¹,Sinagra Gianfranco¹,Weinberger Florian⁵⁶,Eschenhagen Thomas⁵⁶^ORCID,Carrier Lucie⁵⁶^ORCID,Kehat Izhak⁷^ORCID,Tocchetti Carlo G⁸⁹^ORCID,Russo Michele⁸¹⁰^ORCID,Ghigo Alessandra¹⁰^ORCID,Cimino James¹⁰,Hirsch Emilio¹⁰^ORCID,Dawson Dana¹¹^ORCID,Ciccarelli Michele¹²^ORCID,Oliveti Marco¹²,Linke Wolfgang A¹³,Cuijpers Ilona¹⁴¹⁵,Heymans Stephane¹⁴¹⁵,Hamdani Nazha¹⁶¹⁷^ORCID,de Boer Martine¹⁸,Duncker Dirk J¹⁸^ORCID,Kuster Diederik¹⁹^ORCID,van der Velden Jolanda¹⁹^ORCID,Beauloye Christophe⁴²⁰,Bertrand Luc⁴,Mayr Manuel²¹^ORCID,Giacca Mauro¹²²¹,Leuschner Florian²²²³^ORCID,Backs Johannes²²²³,Thum Thomas²⁴²⁵^ORCID

Affiliation:

1. Department of Medicine, Surgery and Health Sciences and Cardiovascular Department, Centre for Translational Cardiology, Azienda Sanitaria Universitaria Giuliano Isontina, strada di Fiume 447, 34149 Trieste (TS), Italy

2. International Center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy

3. Cardiovascular Research and Development Center, Faculty of Medicine, University of Porto, Porto, Portugal

4. Pole of Cardiovascular Research, Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain (UCLouvain), Belgium, Brussels

5. Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

6. DZHK (German Centre for Cardiovascular Research), partner site Hamburg/Kiel/Lübeck, Germany

7. Department of Physiology, Biophysics and System Biology, The Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, Israel

8. Department of Translational Medical Sciences, Federico II University, Naples, Italy

9. Interdepartmental Center of Clinical and Translational Research (CIRCET), Federico II University, Naples, Italy

10. Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy

11. School of Medicine and Dentistry, University of Aberdeen, Aberdeen, UK

12. University of Salerno, Salerno, Italy

13. Institute of Physiology 2, University of Muenster, Muenster, Germany

14. Maastricht University Medical Centre, Maastricht University, Maastricht, The Netherlands

15. Center of Molecular and Vascular Biology (CMVB), KU Leuven, Leuven, Belgium

16. Department of Molecular and Experimental Cardiology, Division Cardiology, St. Josef-Hospital, Ruhr University Bochum, Bochum, Germany

17. Institute of Physiology, Ruhr University Bochum, Bochum, Germany

18. Division of Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands

19. Department of Physiology, Amsterdam UMC, Vrije Universiteit, Amsterdam Cardiovascular Sciences Institute, Amsterdam, The Netherlands

20. Division of Cardiology, Cliniques Universitaires Saint-Luc, Brussels, Belgium

21. King’s College London, British Heart Foundation Centre of Research Excellence, School of Cardiovascular Medicine & Sciences, London, UK

22. Institute of Experimental Cardiology, Department of Cardiology, Angiology & Pulmology, Heidelberg University Hospital, Heidelberg, Germany

23. DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg, Germany

24. Institute for Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Hannover, Germany

25. REBIRTH Center for Translational Regenerative Medicine, Hannover Medical School, Hannover, Germany

Abstract

Abstract Echocardiography is a reliable and reproducible method to assess non-invasively cardiac function in clinical and experimental research. Significant progress in the development of echocardiographic equipment and transducers has led to the successful translation of this methodology from humans to rodents, allowing for the scoring of disease severity and progression, testing of new drugs, and monitoring cardiac function in genetically modified or pharmacologically treated animals. However, as yet, there is no standardization in the procedure to acquire echocardiographic measurements in small animals. This position paper focuses on the appropriate acquisition and analysis of echocardiographic parameters in adult mice and rats, and provides reference values, representative images, and videos for the accurate and reproducible quantification of left ventricular function in healthy and pathological conditions.

Funder

AIRC IG

FEDER

Compete 2020—Programa Operacional Competitividade E Internacionaliza

European Regional Development Fund

NETDIAMOND

European Structural And Investment Funds

Cliniques Universitaires Saint-Luc

Action de Recherche Concertée

ERA-Net-CVD

IMI2-CARDIATEAM

DZHK