A new Antarctic species of Orchomenella G.O. Sars, 1890 (Amphipoda: Lysianassoidea: Tryphosidae): is phase-contrast micro-tomography a mature technique for digital holotypes?

Author:

Giulianini Piero G1ORCID,De Broyer Claude2,Hendrycks Ed A3,Greco Samuele1,D’Agostino Elisa1,Donato Sandro456,Giglio Anita7,Gerdol Marco1,Pallavicini Alberto1ORCID,Manfrin Chiara1

Affiliation:

1. Department of Life Sciences, University of Trieste , via Giorgieri, 34127, Trieste , Italy

2. Royal Belgian Institute of Natural Sciences , rue Vautier 29, B-1000, Bruxelles , Belgium

3. Canadian Museum of Nature, Research and Collections , PO Box 3443, Station D, Ottawa, ON, K1P 6P4 , Canada

4. Department of Physics, University of Calabria , via Bucci, 87036, Arcavacata di Rende, Cosenza , Italy

5. Istituto Nazionale di Fisica Nucleare, Division of Frascati , Via Fermi, 54, 00044, Frascati, Rome , Italy

6. Elettra-Sincrotrone Trieste SCpA , Strada Statale 14 – km 163, 5 in AREA Science Park, 34149, Basovizza, Trieste , Italy

7. Department of Biology, Ecology and Earth Science, University of Calabria , via Bucci, 87036, Arcavacata di Rende, Cosenza , Italy

Abstract

Abstract The purpose of this paper is to describe a new species of Antarctic amphipod of the genus Orchomenella Sars, 1890, Orchomenella rinamontiae sp. nov., and to investigate whether high-resolution images of the surface anatomy obtained ‘in situ’ with synchrotron radiation X-ray phase-contrast micro-tomography (SR-PhC micro-CT) can replace classical approaches to describe a new species. The phylogenetic analyses based on the gene COI support the morphologically based taxonomic assignment. The SR-PhC micro-CT was useful for viewing the three-dimensional reconstructions, with the great advantages that the specimen could be rotated around all axes and that it was possible digitally to remove sections of the image that might have obscured areas of the amphipod on which we were focusing. However, it is not yet a completely reliable technique to describe a new species fully. Classical descriptions using light microscopy and scanning electron microscopy are still necessary. Nevertheless, SR-PhC micro-CT is a promising technique that has the potential to revolutionize the way we study biological samples, accelerating the study of biodiversity.

Funder

Italian Program of Antarctic Research

Publisher

Oxford University Press (OUP)

Reference46 articles.

1. Die Gammaridea (Crustacea: Amphipoda) der Deutschen Antarktis-Expeditionen 1975/76 und 1977/78. 3. Lysianassidae;Andres;Mitteilungen aus den Hamburgischen Zoologischen Museum und Institut,1983

2. The families and genera of marine gammaridean Amphipoda (except marine gammaroids);Barnard;Records of the Australian Museum,1991

3. Phase-contrast breast CT: the effect of propagation distance;Brombal;Physics in Medicine & Biology,2018

4. SYRMEP Tomo Project: a graphical user interface for customizing CT reconstruction workflows;Brun;Advanced Structural and Chemical Imaging,2017

5. “COI-like” sequences are becoming problematic in molecular systematic and DNA barcoding studies;Buhay;Journal of Crustacean Biology,2009

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3