Identification of C. auris clade 5 isolates using claID

Author:

Narayanan Aswathy1ORCID,Selvakumar Pavitra23,Siddharthan Rahul23ORCID,Sanyal Kaustuv1

Affiliation:

1. Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research , Bangalore ,560064, India

2. Computational Biology, The Institute of Mathematical Sciences , Chennai, 600113 , India

3. Homi Bhabha National Institute , Mumbai, 400094 , India

Abstract

Abstract Candida auris poses threats to the global medical community due to its multidrug resistance, ability to cause nosocomial outbreaks and resistance to common sterilization agents. Different variants that emerged at different geographical zones were classified as clades. Clade-typing becomes necessary to track its spread, possible emergence of new clades, and to predict the properties that exhibit a clade bias. We previously reported a colony-Polymerase Chain Reaction-based, clade-identification method employing whole genome alignments and identification of clade-specific sequences of four major geographical clades. Here, we expand the panel by identifying clade 5 which was later isolated in Iran, using specific primers designed through in silico analyses.

Funder

Indian Council of Medical Research

Jawaharlal Nehru Centre for Advanced Scientific Research

Department of Atomic Energy, Government of India

Institute of Mathematical Sciences

Amity University

Publisher

Oxford University Press (OUP)

Reference20 articles.

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