State of art and best practices for fatty acid analysis in aquatic sciences-Reference-Cited by-同舟云学术

State of art and best practices for fatty acid analysis in aquatic sciences

Published:2020-08-23 Issue:7-8 Volume:77 Page:2375-2395
ISSN:1095-9289
Container-title:ICES Journal of Marine Science
language:en
Short-container-title:

Author:

Couturier Lydie I E¹^ORCID,Michel Loïc N²^ORCID,Amaro Teresa³,Budge Suzanne M⁴,da Costa Elisabete⁵,De Troch Marleen⁶,Di Dato Valeria⁷,Fink Patrick⁸^ORCID,Giraldo Carolina⁹,Le Grand Fabienne¹,Loaiza Iván⁶,Mathieu-Resuge Margaux¹⁰,Nichols Peter D¹¹,Parrish Christopher C¹²^ORCID,Sardenne Fany¹,Vagner Marie¹,Pernet Fabrice¹^ORCID,Soudant Philippe¹

Affiliation:

1. Univ Brest, Ifremer, CNRS, IRD, LEMAR, Plouzané F-29280, France

2. Ifremer, Centre de Bretagne, REM/EEP, Laboratoire Environnement Profond, Plouzané 29280, France

3. Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto (U.Porto), Av. General Norton de Matos s/n, Matosinhos 4450-208, Portugal

4. Department of Process Engineering and Applied Science, Dalhousie University, 5273 DaCosta Row, Halifax, NS B3H 4R2, Canada

5. CESAM - Centre for Environmental and Marine Studies & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, Aveiro 3810-193, Portugal

6. Department of Biology, Marine Biology, Ghent University, Krijgslaan 281/S8, Ghent 9000, Belgium

7. Marine Biotechnology Department, Stazione Zoologica Anton Dohrn Napoli, Villa Comunale, Napoli, Italy

8. Helmholtz Centre for Environmental Research – UFZ, Department Aquatic Ecosystem Analysis and Management and Department River Ecology, Brückstrasse 3a, 30114 Magdeburg, Germany

9. Ifremer - Centre Manche Mer du Nord, Département Ressources Biologiques et Environnement, Laboratoire des Ressources Halieutiques, 150 Quai Gambetta, BP 699, Boulogne-sur-Mer 62321, France

10. WasserCluster Lunz – Inter-University Center for Aquatic Ecosystems Research, Lunz am See 3293, Austria

11. CSIRO Oceans and Atmosphere, GPO Box 1538, Hobart, TAS 7000, Australia

12. Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, Newfoundland A1C 5S7, Canada

Abstract

Abstract Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.

Funder

National Research Organizations

University of Brest

European Institute for Marine Studies

European Marine Research Network

“Laboratoire d'Excellence” LabexMER

CESAM

Publisher

Oxford University Press (OUP)

Subject

Ecology,Aquatic Science,Ecology, Evolution, Behavior and Systematics,Oceanography

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