Report of the Assay Guidance Workshop on 3-Dimensional Tissue Models for Antiviral Drug Development

Author:

Jordan Robert1,Ford-Scheimer Stephanie L2,Alarcon Rodolfo M3,Atala Anthony4ORCID,Borenstein Jeffrey T5ORCID,Brimacombe Kyle R2ORCID,Cherry Sara6ORCID,Clevers Hans7,Davis Mindy I3ORCID,Funnell Simon G P89ORCID,Gehrke Lee1011,Griffith Linda G1213,Grossman Abigail C2,Hartung Thomas1415ORCID,Ingber Donald E11161718,Kleinstreuer Nicole C19,Kuo Calvin J20,Lee Emily M2,Mummery Christine L21ORCID,Pickett Thames E3,Ramani Sasirekha22ORCID,Rosado-Olivieri Edwin A23ORCID,Struble Evi B24ORCID,Wan Zhengpeng12ORCID,Williams Mark S3,Hall Matthew D2ORCID,Ferrer Marc2,Markossian Sarine2ORCID

Affiliation:

1. Bill and Melinda Gates Foundation , Seattle, Washington , USA

2. National Center for Advancing Translational Sciences, National Institutes of Health , Rockville, Maryland , USA

3. National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland , USA

4. Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA

5. Draper Laboratory , Cambridge, Massachusetts , USA

6. Department of Pathology and Laboratory Medicine, University of Pennsylvania , Philadelphia, Pennsylvania , USA

7. Hubrecht Institute , Utrecht , The Netherlands

8. UK Health Security Agency , Salisbury , United Kingdom

9. Quadram Institute Bioscience, Norwich, United Kingdom

10. Institute for Medical Engineering and Science, Massachusetts Institute of Technology , Cambridge, Massachusetts , USA

11. Harvard Medical School , Boston, Massachusetts , USA

12. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

13. Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

14. Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University , Baltimore, Maryland , USA

15. Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA

16. Wyss Institute for Biologically Inspired Engineering, Harvard University , Boston, Massachusetts , USA

17. Harvard School of Engineering and Applied Sciences , Cambridge, Massachusetts , USA

18. Boston Children's Hospital , Boston, Massachusetts , USA

19. National Institute of Environmental Health Sciences, National Institutes of Health , Research Triangle, North Carolina , USA

20. Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, California, USA

21. Leiden University Medical Center , Leiden , The Netherlands

22. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA

23. RumiViro, RUMI Scientific, Inc , New York, New York , USA

24. US Food and Drug Administration , Silver Spring, Maryland , USA

Abstract

Abstract The National Center for Advancing Translational Sciences (NCATS) Assay Guidance Manual (AGM) Workshop on 3D Tissue Models for Antiviral Drug Development, held virtually on 7–8 June 2022, provided comprehensive coverage of critical concepts intended to help scientists establish robust, reproducible, and scalable 3D tissue models to study viruses with pandemic potential. This workshop was organized by NCATS, the National Institute of Allergy and Infectious Diseases, and the Bill and Melinda Gates Foundation. During the workshop, scientific experts from academia, industry, and government provided an overview of 3D tissue models’ utility and limitations, use of existing 3D tissue models for antiviral drug development, practical advice, best practices, and case studies about the application of available 3D tissue models to infectious disease modeling. This report includes a summary of each workshop session as well as a discussion of perspectives and challenges related to the use of 3D tissues in antiviral drug discovery.

Funder

NCATS

National Institutes of Health

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

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