Fluorometric Determination of Total and Bound Sulfite in Wine by N-(9-Acridinyl)maleimide

Author:

Akasaka Kazuaki1,Ohrui Hiroshi1,Meguro Hiroshi1,Suzuki Tateo2,Miwa Harufumi3

Affiliation:

1. Tohoku University, Faculty of Agriculture, 1-1 Tsutsumidori-Amamiyamachi, Aoba, Sendai 981, Japan

2. National Food Research Institute, 2-1-2 Kannondai, Tsukuba 305, Japan

3. Ajinomoto Technical Center, 1-1 Suzukicho, Kawasaki, Kawasaki 210, Japan

Abstract

Abstract N-(9-Acridinyl)maleimide (NAM) reacts with sulfite in wine and gives strong fluorescent derivatives that lead to highly sensitive fluorometry of both total and bound sulfite in wine. Values of free and bound sulfite in wine determined by the NAM method and the modified Rankine method agreed. Sulfite was determined in <200 μL wine within 2 h.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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