Planar Chromatographic and Liquid Chromatographic Analysis of Thiamphenicol in Bovine and Human Plasma

Author:

Dreassi Elena1,Corbini Gianfranco1,Ginanneschi Vittoria1,Corti Piero1,Furlanetto Sandra2

Affiliation:

1. Università di Siena, Dipartimento Farmaco Chimico Tecnologico, Via Banchi di Sotto 55, 53100 Siena, Italy

2. Università di Firenze, Dipartimento di Scienze Farmaceutiche, Via G. Capponi 9, 50121 Florence, Italy

Abstract

Abstract A planar chromatographic (PC) method was developed to determine thiamphenicol in human and bovine plasma with florphenicol as internal standard. The performance of the method was compared with that of a liquid chromatographic method. Recovery of extraction method developed for plasma was 81.51 ± 2.85%. Reproducibilities of the PC assay performed with various PC plates were also good.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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