RNase H1 facilitates recombinase recruitment by degrading DNA–RNA hybrids during meiosis

Author:

Liu Chao12,Wang Liying1,Li Yanan3ORCID,Guo Mengmeng24,Hu Jun5,Wang Teng3,Li Mengjing6,Yang Zhuo7,Lin Ruoyao5,Xu Wei7ORCID,Chen Yinghong24,Luo Mengcheng8ORCID,Gao Fei24ORCID,Chen Jia-Yu5,Sun Qianwen79ORCID,Liu Hongbin6ORCID,Sun Bo3ORCID,Li Wei124ORCID

Affiliation:

1. Guangzhou Women and Children's Medical Center, Guangzhou Medical University , Guangzhou  510623, China

2. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences , Beijing  100101, China

3. School of Life Science and Technology, ShanghaiTech University , Shanghai  201210, China

4. University of Chinese Academy of Sciences , Beijing  100049, China

5. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University , Nanjing  210023, China

6. Center for Reproductive Medicine, Shandong University , Jinan  250012, China

7. Center for Plant Biology, School of Life Sciences, Tsinghua University , Beijing  100084, China

8. Department of Tissue and Embryology, School of Basic Medical Sciences, Wuhan University, Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan  430072, China

9. Tsinghua-Peking Center for Life Sciences , Beijing  100084, China

Abstract

Abstract DNA–RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme that degrades the RNA within DNA–RNA hybrids, impairs spermatogenesis and causes male infertility. Notably, Rnaseh1 knockout results in incomplete DNA repair and meiotic prophase I arrest. These defects arise from the altered RAD51 and DMC1 recruitment in zygotene spermatocytes. Furthermore, single-molecule experiments show that RNase H1 promotes recombinase recruitment to DNA by degrading RNA within DNA–RNA hybrids and allows nucleoprotein filaments formation. Overall, we uncover a function of RNase H1 in meiotic recombination, during which it processes DNA–RNA hybrids and facilitates recombinase recruitment.

Funder

National Natural Science Foundation of China

National Science Fund for Distinguished Young Scholars

Natural Science Foundation of Shanghai

National Key Research and Development Program of China

Science and Technology Project of Guangzhou

Youth Talent Support Programme of Guangdong Provincial Association for Science and Technology

Publisher

Oxford University Press (OUP)

Subject

Genetics

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3