T5-like phage BF23 evades host-mediated DNA restriction and methylation

Author:

Skutel Mikhail1,Andriianov Aleksandr1,Zavialova Maria12,Kirsanova Maria1,Shodunke Oluwasefunmi13,Zorin Evgenii1,Golovshchinskii Aleksandr4,Severinov Konstantin5ORCID,Isaev Artem1ORCID

Affiliation:

1. Skolkovo Institute of Science and Technology , Bolshoy Boulevard 30/1, 143028, Moscow , Russia

2. Institute of Biomedical Chemistry (IBMC) , Pogodinskaya 10/8, 119435, Moscow , Russia

3. Moscow Institute of Physics and Technology , Institutskiy Pereulok 9, 141701, Dolgoprudny , Russia

4. School of Pedagogical Skills Center , Olympiyskiy Prospect 11/1, 129272, Moscow , Russia

5. Waksman Institute of Microbiology , 190 Frelinghuysen Rd, NJ 08854, Piscataway , United States

Abstract

Abstract Bacteriophage BF23 is a close relative of phage T5, a prototypical Tequintavirus that infects Escherichia coli. BF23 was isolated in the middle of the XXth century and was extensively studied as a model object. Like T5, BF23 carries long ∼9.7 kb terminal repeats, injects its genome into infected cell in a two-stage process, and carries multiple specific nicks in its double-stranded genomic DNA. The two phages rely on different host secondary receptors—FhuA (T5) and BtuB (BF23). Only short fragments of the BF23 genome, including the region encoding receptor interacting proteins, have been determined. Here, we report the full genomic sequence of BF23 and describe the protein content of its virion. T5-like phages represent a unique group that resist restriction by most nuclease-based host immunity systems. We show that BF23, like other Tequintavirus phages, resist Types I/II/III restriction–modification host immunity systems if their recognition sites are located outside the terminal repeats. We also demonstrate that the BF23 avoids host-mediated methylation. We propose that inhibition of methylation is a common feature of Tequintavirus and Epseptimavirus genera phages, that is not, however, associated with their antirestriction activity.

Funder

Ministry of Science and Higher Education of the Russian Federation

RSF

Publisher

Oxford University Press (OUP)

Subject

General Medicine

Reference101 articles.

1. The anatomy of the T5 bacteriophage DNA molecule;Abelson;J Mol Biol,1966

2. Novel site-specific endonucleases F-TflI, F-TflII and F-TflIV encoded by the bacteriophage T5;Akulenko;Molekuliarnaia Biologiia,2004

3. Phage T3 overcomes the BREX defence through SAM cleavage and inhibition of SAM synthesis;Andriianov,2023

4. Viruses encode tRNA and anti-retron to evade bacterial immunity;Azam,2023

5. Nuclease genes occupy boundaries of genetic exchange between bacteriophages;Barth,2023

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3