Author:
Azam Aa Haeruman,Chihara Kotaro,Kondo Kohei,Nakamura Tomohiro,Ojima Shinjiro,Tamura Azumi,Yamashita Wakana,Cui Longzhu,Takahashi Yoshimasa,Watashi Koichi,Kiga Kotaro
Abstract
AbstractRetrons are bacterial genetic retroelements that encode reverse transcriptase capable of producing multicopy single-stranded DNA (msDNA) and function as antiphage defense systems. Phages employ several strategies to counter the host defense systems, but no mechanisms for evading retrons are known. Here, we show that tRNATyrand Rad (retron anti-defense) of T5 phage family inhibit the defense activity of retron 78 and a broad range of retrons, respectively. The effector protein of retron 78, ptuAB, specifically degraded tRNATyrleading abortive infection, but phage countervailed this defense by supplying tRNATyr. Rad inhibited retron function by degrading noncoding RNA, the precursor of msDNA. In summary, we demonstrated that viruses encode at least two independent strategies for overcoming bacterial defense systems: anti-defense, such as Rad, and defense canceler, like tRNA.
Publisher
Cold Spring Harbor Laboratory
Cited by
15 articles.
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